"THIS IS THE GREATEST HIGH!" —DIO after ingesting the blood of Joseph
The vial of Joestar blood is a small glass vial of blood from supposedly Joseph Joestar, which is earned after completing the quest to evolve Shadow DIO to The World. If the user uses this while equipped with Shadow DIO, they'll drink the glass and evolve to The World.
You can obtain this item by talking to Him (NPC) at the forest with shadow dio and completing his quest.
- In the anime/manga, there never was an actual vial of Joseph's blood but DIO straight up drinking the blood from Joseph's corpse.
- Upon using this item on shadow dio will have a 2-3 secs windup.